Biotinylated Human MICB Protein Recombinant
Category: Biotinylated proteins$542.00 – $1,805.00
Description
Accession
Q29980
Source
DNA sequence encoding Human MICB including a C-His-Avi tag was expressed in HEK293 Cells.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
Molecular weight
Recombinant Human MICB is a protein consisting of amino acid residues [Ala23-Gly298] ,The protein has a predicted MW of 34.4 kDa. Due to glycosylation, the protein migrates to 48-65 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human MICB was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Biological Process
Biological Process
Biological Process
Methods
Biotinylated Human MICB Protein Tris-Bis PAGE
- Biotinylated Human MICB on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%.
Biotinylated Human MICB Protein SEC-HPLC
- The purity of Biotinylated Human MICB is greater than 95% as determined by SEC-HPLC.
Biotinylated Human MICB Protein SPR Data
- Human NKG2D, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human MICB, His Tag with an affinity constant of 60.38 nM as determined in SPR assay (Biacore T200).
