Biotinylated Human IL-1R3/IL-1 RAcP Protein Recombinant
Category: Biotinylated proteins$542.00 – $1,805.00
Description
Accession
Q9NPH3
Source
DNA sequence encoding Human IL-1R3/IL-1 RAcP including a C-His-Avi tag was expressed in HEK293 Cells.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
Molecular weight
Recombinant Human IL-1R3/IL-1 RAcP is a protein consisting of amino acid residues [Ser21-Glu359] ,The protein has a predicted MW of 42 kDa. Due to glycosylation, the protein migrates to 52-70 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human IL-1R3/IL-1 RAcP was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Interactor
Biological Process
Biological Process
Molecular function
Methods
Biotinylated Human IL-1R3/IL-1 RAcP Protein Tris-Bis PAGE
- Biotinylated Human IL-1R3 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Biotinylated Human IL-1R3/IL-1 RAcP Protein SEC-HPLC
- The purity of Biotinylated Human IL-1R3 is greater than 95% as determined by SEC-HPLC.
Biotinylated Human IL-1R3/IL-1 RAcP Protein ELISA Data
- Immobilized Biotinylated Human IL-1R3 at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-IL-1R3 Antibody, hFc Tag with the EC50 of 11.3ng/ml determined by ELISA.
