Biotinylated Human GPC3/Glypican 3 Protein Recombinant
Category: Biotinylated proteins$542.00 – $1,805.00
Description
Accession
P51654
Source
DNA sequence encoding Human GPC3/Glypican 3 including a C-His-Avi tag was expressed in HEK293 Cells.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
Molecular weight
Recombinant Human GPC3/Glypican 3 is a protein consisting of amino acid residues [Gln25-His559] ,The protein has a predicted MW of 63.6 kDa. Due to glycosylation, the protein migrates to 42 kDa, 80-135 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human GPC3/Glypican 3 was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Molecular function
Methods
Biotinylated Human GPC3/Glypican 3 Protein Tris-Bis PAGE
- Biotinylated Human GPC3 on Tris-Bis PAGE under reduced condition. The purity is greater than 90%.
Biotinylated Human GPC3/Glypican 3 Protein ELISA Data
- Immobilized Anti-GPC3 Antibody, hFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human GPC3, His Tag with the EC50 of 10.8ng/ml determined by ELISA.
