Biotinylated Human GPA33/A33 Protein Recombinant
Category: Biotinylated proteins$542.00 – $1,805.00
Description
Accession
Q99795
Source
DNA sequence encoding Human GPA33/A33 including a C-His-Avi tag was expressed in HEK293 Cells.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
Molecular weight
Recombinant Human GPA33/A33 is a protein consisting of amino acid residues [Ile22-Val235] ,The protein has a predicted MW of 26.5 kDa. Due to glycosylation, the protein migrates to 28-40 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human GPA33/A33 was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Methods
Biotinylated Human GPA33/A33 Protein Tris-Bis PAGE
- Biotinylated Human GPA33 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Biotinylated Human GPA33/A33 Protein SEC-HPLC
- The purity of Biotinylated Human GPA33 is greater than 95% as determined by SEC-HPLC.
Biotinylated Human GPA33/A33 Protein ELISA Data
- Immobilized Anti-GPA33 Antibody, hFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human GPA33, His Tag with the EC50 of 11.0ng/ml determined by ELISA.
