Biotinylated Human GFRAL/GFR alpha-like Protein Recombinant
Category: Biotinylated proteins$542.00 – $1,805.00
Description
Accession
Q6UXV0
Source
DNA sequence encoding Human GFRAL/GFR alpha-like including a C-His-Avi tag was expressed in HEK293 Cells.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
Molecular weight
Recombinant Human GFRAL/GFR alpha-like is a protein consisting of amino acid residues [Ser19-Glu351] ,The protein has a predicted MW of 40.7 kDa. Due to glycosylation, the protein migrates to 48-62 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human GFRAL/GFR alpha-like was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Methods
Biotinylated Human GFRAL/GFR alpha-like Protein Tris-Bis PAGE
- Biotinylated Human GFRAL on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%.
Biotinylated Human GFRAL/GFR alpha-like Protein SEC-HPLC
- The purity of Biotinylated Human GFRAL is greater than 95% as determined by SEC-HPLC.
Biotinylated Human GFRAL/GFR alpha-like Protein ELISA Data
- Immobilized Human GDF15, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human GFRAL, His Tag with the EC50 of 0.1μg/ml determined by ELISA.
Biotinylated Human GFRAL/GFR alpha-like Protein Blocking Data
- Serial dilutions of Anti-GDF15 Antibody were added into Human GDF15, His Tag : Biotinylated Human GFRAL, His Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 40.8ng/ml.
