//Biotinylated Human FcRH5/FcRL5 Protein Recombinant

Biotinylated Human FcRH5/FcRL5 Protein Recombinant

Category:

$542.00$1,805.00

SKU: KT7JDF8HB

Description

Accession
Q96RD9
Source
DNA sequence encoding Human FcRH5/FcRL5 including a C-His-Avi tag was expressed in HEK293 Cells.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
Molecular weight
Recombinant Human FcRH5/FcRL5 is a protein consisting of amino acid residues [Gln16-Gly851] ,The protein has a predicted MW of 94.2 kDa. Due to glycosylation, the protein migrates to 110-120 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human FcRH5/FcRL5 was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

Methods

Biotinylated Human FcRH5/FcRL5 Protein Tris-Bis PAGE

  • Biotinylated Human FcRH5 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Biotinylated Human FcRH5/FcRL5 Protein ELISA Data

  • Immobilized Anti-FcRH5 Antibody at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human FcRH5, His Tag with the EC50 of 0.23μg/ml determined by ELISA.
Stay in the loop
For new product information and regular updates on our product range please fill your details
Loading