Biotinylated Human EGFR/HER1 Protein Recombinant
Category: Biotinylated proteins$542.00 – $1,805.00
Description
Accession
P00533
Source
DNA sequence encoding Human EGFR/HER1 including a C-His-Avi tag was expressed in HEK293 Cells.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
Molecular weight
Recombinant Human EGFR/HER1 is a protein consisting of amino acid residues [Leu25-Ser645] ,The protein has a predicted MW of 71.5 kDa. Due to glycosylation, the protein migrates to 90-120 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human EGFR/HER1 was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Interactor
O14964
Interactor
Interactor
Interactor
Interactor
P22681
Interactor
P51149
Interactor
P62988
Interactor
P62991
Interactor
Q13596
Interactor
Q9UKV8
Interactor
Q9UPY3
Interactor
Interactor
Interactor
Interactor
Interactor
P29353
Interactor
Interactor
P62993
Interactor
Q03135
Interactor
Q13191
Interactor
Q6NZI2
Interactor
Q9UJM3
Molecular function
Molecular function
Molecular function
Methods
Biotinylated Human EGFR/HER1 Protein Tris-Bis PAGE
- Biotinylated Human EGFR/HER1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Biotinylated Human EGFR/HER1 Protein SEC-HPLC
- The purity of Biotinylated Human EGFR/HER1 is greater than 95% as determined by SEC-HPLC.
Biotinylated Human EGFR/HER1 Protein SPR Data
- Biotinylated Human EGFR, His-Avi Tag captured on CM5 Chip via Anti-his antibody can bind Human EGF, No Tag with an affinity constant of 0.64 nM as determined in SPR assay (Biacore T200).
