Human Neuregulin-4 Recombinant
Categories: EGF familyEGF familyRecombinant Human Cytokines$160.00 – $2,000.00
Description
Accession
Q8WWG1
Source
Optimized DNA sequence encoding Human Neuregulin-4 EGF domainwas expressed in Escherichia Coli.
Molecular weight
Recombinant human Neuregulin-4 EGF domain is a monomeric protein consisting of62 amino acid residue subunits, and migrates as an approximately7 kDa protein under non-reducing and reducing conditions in SDS-PAGE.
Purity
>96%, as determined by SDS-PAGE and HPLC
Biological Activity
The ED(50) was determined by the phosphorylation of ErbB2 and ErbB4 receptors in CHO cells, and was found to be <3ng/ml, corresponding to a specific activity ofx Units/mg.
Protein Sequence
MPTDHEEPCG PSHKSFCLNG GLCYVIPTIP SPFCRCVENY TGARCEEVFL PGSSIQTKSN LFEAF VALAV LVTLIIGAFY FLCRKGHFQR ASSVQYDINL VETSSTSAHH SHEQH
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Neuregulin-4was lyophilized from a 0.2 μm filteredmM PB, pH7.0.
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage
The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at2° -8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Molecular function