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Mouse acidic Fibroblast Growth Factor Recombinant

aFGF

accession:  P61148
   Size A     10ug   $ 70
   Size B      50ug  $160
   Size C      1mg  $ 1350
Domain  :  HBGF/IL1
Gene  :  FGF1
Catalog no. :  RKP61148
Source:
Optimized DNA sequence encoding Mouse acidic FGF mature chain was expressed in Escherichia Coli.

 
Molecular weight:

Native Mouse aFGF, generated by the proteolytic removal of the signal peptide
and propeptide, the moleculte has a calculated mass of 16 kDa.

Recombinant FGF acidic is a disulfide-linked monomer  protein consisting of 141 amino acids and migrates as an approximately 16 kDa protein under reducing and non-reducing conditions.


 
Purity:
>95%, as determined by SDS-PAGE and HPLC

 
Biological Activity:
The ED50 as determined by the dose-dependent stimulation of thymidine uptake by BaF3 cells expressing FGF receptors is ≤ 10 ng/ml, corresponding to a specific activity of ≥ 1 x 105 units/mg.
 

 
Protein Sequence:
        10         20         30         40         50         60 
MAEGEITTFA ALTERFNLPL GNYKKPKLLY CSNGGHFLRI LPDGTVDGTR DRSDQHIQLQ 

        70         80         90        100        110        120 
LSAESAGEVY IKGTETGQYL AMDTEGLLYG SQTPNEECLF LERLEENHYN TYTSKKHAEK 

       130        140        150 
NWFVGLKKNG SCKRGPRTHY GQKAILFLPL PVSSD 
(*)Complete precursor sequence shown, expressed chain highlighted
 
Endotoxin:
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

 
Presentation:
Recombinant mouse aFGF was lyophilized from a 0.2 μm filtered PBS solution.

 
Reconstitution:
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

 
Storage:
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity.

Avoid repeated freeze/thaw cycles.

 
Usage:
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
 

aFGF

aFGF and bFGF display 55 % homology which is limited, however, to a few functional domains. The structure of aFGF, revealed by X-ray crystallography, shows a similar folding structure as IL1. Because aFGF binds to the same receptor as bFGF, aFGF displays more or less the same spectrum of activities. It is, however, generally less active than bFGF in similar assays. FGF receptors are encoded by a gene family consisting of at least four receptor tyrosine kinases that transduce signals important in a variety of developmental and physiological processes related to cell growth and differentiation.

Related Publications:
expression of acidic fibroblast growth factor (aFGF) and fibroblast growth factor receptor 4 (fgfr4) in breast fibroadenomas
J. Clin. Pathol., Jan 2001; 54: 37 - 41.
requirement of phosphatidylinositol 3-kinase activity for translocation of exogenous aFGF to the cytosol and nucleus
J. Biol. Chem., Apr 2000; 275: 11972.
angiogenic potential of perivascularly delivered aFGF in a porcine model of chronic myocardial ischemia
Am J Physiol Heart Circ Physiol, Mar 1998; 274: H930 - H936.
the fibroblast growth factor receptor-1 is necessary for the induction of neurite outgrowth in pc12 cells by aFGF
J. Neurosci., Aug 1996; 16: 4579.
the major acidic fibroblast growth factor (aFGF)-stimulated phosphoprotein from bovine liver plasma membranes has aFGF-stimulated kinase, autoadenylylation, and alkaline nucleotide phosphodiesterase activities
J. Biol. Chem., Dec 1993; 268: 27318 - 27326.




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