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Human Interleukin-13 Recombinant


accession:  P35225
   Size A     2ug   $ 70
   Size B      10ug  $160
   Size C      1mg  $ 4700
Domain  :  4 helix cytokine core
Gene  :  IL13
Catalog no. :  RKP35225
Optimized DNA sequence encoding Human Interleukin-13 mature chain was expressed in Escherichia Coli.

Molecular weight:

Native human Interleukin-13, generated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately 13 kDa.

Recombinant IL-13 is a disulfide-linked monomer protein consisting of 115 amino acid residue subunits,and migrates as an approximately 13 kDa protein under non-reducing and reducing conditions in SDS-PAGE.

>95%, as determined by SDS-PAGE and HPLC

Biological Activity:
The ED(50) was determined by the dose-dependent  proliferation of TF-1 cells was ≤ 2.0 ng/ml, corresponding to a specific activity of ≥ 1 x 106 units/mg.

Protein Sequence:
        10         20         30         40         50         60 

        70         80         90        100        110        120 

(*)Complete precursor sequence shown, expressed chain highlighted
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

Recombinant Interleukin-13 was lyophilized from a 0.2μm filtered concentrated (1mg/ml) solution in PBS, pH 7.2.

A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity.

Avoid repeated freeze/thaw cycles.

This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.



Mouse IL13 is produced primarily by activated Th2 cells. Human IL13 is expressed in activated T- helper cells (resembling Th0, Th1, Th2), and T-cells expressing CD8. The alpha chain of the IL13 receptor has weak binding activity for IL13. High affinity receptors are formed when the receptor alpha chains of IL13 and IL4 receptors are coexpressed. The receptors for IL13 and IL4 share a component,a common gamma subunit that is found also in the IL2 receptor.Different IL13 receptor structures have been shown to exist on various cell types and the IL13 receptor may share more than one component with IL4 receptor. IL13 down-modulates macrophage activity, reducing the production of pro-inflammatory cytokines and chemokines in response to IFN-gamma or bacterial lypopolysaccharides. IL13 enhances the production of the IL1 receptor antagonist IL1ra. IL13 also decreases the production of nitric oxide by activated macrophages, leading to a decrease in parasiticidal activity. IL13 induces differentiation of human monocytes, enhances survival time in culture, and also induces differentiation and proliferation and Isotype switching in B-cells.

Related Publications:
IL-13-induced oxidative stress via microglial nadph oxidase contributes to death of hippocampal neurons in vivo
J. Immunol., Oct 2009; 183: 4666 - 4674.
involvement of IL-13 in tobacco smoke induced changes in the structure and function of rat intrapulmonary airways
Am. J. Respir. Cell Mol. Biol., Sep 2009; 10.1165/rcmb.2009-0117OC.
disruption of th2 immunity results in a gender-specific expansion of IL-13 producing accessory nk cells during helminth infection
J. Immunol., Sep 2009; 183: 3906 - 3914.
distinct structural requirements for interleukin-4 (il-4) and IL-13 binding to the shared IL-13 receptor facilitate cellular tuning of cytokine responsiveness
J. Biol. Chem., Sep 2009; 284: 24289 - 24296.
IL-13 receptor 2 regulates the immune and functional response to nippostrongylus brasiliensis infection
J. Immunol., Aug 2009; 183: 1934 - 1939.

by class:
Growth factors
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by species:
Purified Polyclonal
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