Description
Accession
P56478
Source
Optimized DNA sequence encoding Rat IL7 mature chain was expressed in ECOLI. Recombinant Rat Interleukin-7 encodes amino acid residues 26-154.
Molecular weight
Recombinant IL7 migrates as an approximately 13 kDa protein under reducing conditions in SDS-PAGE.
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
Determined by proliferation of murine CTLL-2 cells is < 5.0 ng/ml, corresponding to a specific activity of > 2 x 10^5 units/mg.
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
IL7 was Lyophilized from PBS, pH 7.2.
Sequence
MNWIDVRYDL EKIESLIQSI HIDTTLYTDS DFHPSCKVTA MNCFLLELQV ILHEYSNMTL NETVRNVLYL ANSTLSSNKN VAESGCKECE ELEEKTFTEF LQSFIRIVQM FINTS
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C. Upon reconstitution, this product can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Molecular function
Molecular function
