///Mouse MEC (CCL28 ) Recombinant

Mouse MEC (CCL28 ) Recombinant

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$70.00$2,700.00

SKU: RKQ9JIL2 Tags: , ,

Description

Accession
Q9JIL2
Source
Optimized DNA sequence encoding mouse CCL28 mature chain was expressed in Escherichia Coli.
Molecular weight
Native mouse MEC/CCL28 has a calculated molecular mass of approximately 12 kDa. Recombinant CCL28 is a monomer protein consisting of 111 amino acid residue subunits, andmigrates as an approximately 12 kDa protein under reducing conditions in SDS-PAGE.
Purity
>97%, as determined by SDS-PAGE and HPLC
Biological Activity
Determined by the ability to chemoattract human lymphocytes using a concentration range of.0-10.0 ng/ml.

Protein Sequence
MQQAGLTLMA VAVCVAFQTS EAILPMASSC CTEVSHHVSG RLLERVSSCS IQRADGDCDL AAVILHVKRR RICISPHNRT LKQWMRASEV KKNGRENVCS GKKQPSRKDR KGHTTRKHRT RGTHRHEASR
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg).
Presentation
Recombinant CCL-28 was lyophilized from PBS pH.4.
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers.
Storage
Upon reconstitution, this cytokine can be stored in working aliquots at° -° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Biological Process
Molecular function

Methods

Non-competitive CCR7-mediated chemotaxis assay of CD11c+ lung cells

  • The chemotaxis assay was performed as previously described 5 CD11c+ lung cells in a total volume of 100 µl chemotaxis medium (RPMI-1640, 0.5% BSA) was added to the upper chambers of 24-well transwell plates with 5-µm-pore-size polycarbonate inserts .
  • 600 µl of chemotaxis medium containing either CCL19 (100 ng/ml, Shenandoah Biotechnology, Warwick, PA), CCL21 (100 ng/ml, , ) or CCL28 (100 ng/ml) were added to the lower chambers.
  • After 5 hours of incubation at 37°C, migrated cells from the lower chambers were collected, stained with anti-CD11c and anti-MHC-II , and enumerated with Trucount beads by flow cytometry ( FACS ).
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