Optimized DNA sequence encoding Human TNFRSF10A mature chain was expressed in ECOLI. Recombinant Human Tumor necrosis factor receptor superfamily member 10A encodes amino acid residues 24-468.
Recombinant TNFRSF10A migrates as an approximately 24 kDa protein under reducing conditions in SDS-PAGE.
>95%, as determined by SDS-PAGE and HPLC
Measured by its ability to inhibit apoptosis in LN-18 cells. The expected ED50 for this effect is 0.4 -0.5 Âµg/ml.
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
TNFRSF10A was Lyophilized from PBS, pH 7.2.
SGTGAAAATP SKVWGSSAGR IEPRGGGRGA LPTSMGQHGP SARARAGRAP GPRPAREASP RLRVHKTFKF VVVGVLLQVV PSSAATIKLH DQSIGTQQWE HSPLGELCPP GSHRSERPGA CNRCTEGVGY TNASQQLFAC LPCTACKSDE EERSPCTTTR NTACQCKPGT FRNDNSAEMC RKCSTGCPRG MVKVKDCTPW SDIECVHKES GNGHN
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C. Upon reconstitution, this product can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.