Description
Accession
Q13421
Source
Optimized DNA sequence encoding Human MSLN mature chain was expressed in ECOLI. Recombinant Human Mesothelin encodes amino acid residues 37-606.
Molecular weight
Recombinant MSLN migrates as an approximately 28 kDa protein under reducing conditions in SDS-PAGE.
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
MSLN was Lyophilized from PBS, pH 7.2.
Sequence
LAGETGQEAA PLDGVLANPP NISSLSPRQL LGFPCAEVSG LSTERVRELA VALAQKNVKL STEQLRCLAH RLSEPPEDLD ALPLDLLLFL NPDAFSGPQA CTRFFSRITK ANVDLLPRGA PERQRLLPAA LACWGVRGSL LSEADVRALG GLACDLPGRF VAESAEVLLP RLVSCPGPLD QDQQEAARAA LQGGGPPYGP PSTWSVSTMD ALRGLLPVLG QPIIRSIPQG IVAAWRQRSS RDPSWRQPER
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C. Upon reconstitution, this product can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Biological Process
