Human Endocrine Gland Vascular Endothelial Growth Factor Recombinant
Category: Recombinant Human Cytokines$70.00 – $2,700.00
Description
Accession
P58294
Source
Optimized DNA sequence encoding Human EG-VEGF mature chain was expressed in Escherichia Coli.
Molecular weight
Native human EG-VEGF is generated by the proteolytic removal of the signal peptide, This molecule has a calculated molecular mass of approximately 10 kDa. Recombinant EG-VEGF is a monomer protein consisting of 86 amino acid residue subunits,and migrates as an approximately 10 kDa protein under reducing conditions in SDS-PAGE.
Purity
>98%, as determined by SDS-PAGE and HPLC
Protein Sequence
MRGATRVSIM LLLVTVSDCA VITGACERDV QCGAGTCCAI SLWLRGLRMC TPLGREGEEC HPGSHKVPFF RKRKHHTCPC LPNLLCSRFP DGRYRCSMDL KNINF
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant EG-VEGFwas lyophilized from a 0.2 μm filtered PBS solution.
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage
The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at2° -8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Biological Process
Molecular function
Molecular function
Methods
Expression of PROK1 and PROKR1 mRNA in human uteroplacental tissues at term.
- PROK1 mRNA expression is elevated in myometrium and placenta during labor at term compared with the tissues collected at term before the onset of labor .
