Human BCMA (CD269) Recombinant
Category: Surface & Soluble Receptors$160.00 – $3,300.00
Description
Accession
Q02223
Source
Optimized DNA sequence encoding Human BCMA extracellular domain sp|Q02223|1-54 fused to C terminal Human IgG1 Fc His tag was expressed in HEK293 Cells.
Molecular weight
Recombinant BCMA (CD269) Fc chimera is a homodimer protein consisting of 300 amino acid residue subunits,and migrates as an approximately 80kDa band under non-reducing and ask 40Da band under reducing conditions in SDS-PAGE.
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
The ED(50) was determined by the ability to bind immobilized recombinant Human BAFF and was calculated to beng/ml .
Protein Sequence
MLQMAGQCSQ NEYFDSLLHA CIPCQLRCSS NTPPLTCQRY CNASVTNSVK GTNA ILWTCL
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant BCMA was lyophilized from a 0.2 μm filteredmm Tris solutionpH.5.
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage
The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this product can be stored in working aliquots at2° -8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Biological Process
Molecular function
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Production system:yeast
