Description
Accession
P16035
Source
Optimized DNA sequence encoding mature chain of human Metalloproteinase inhibitor 2 -TIMP-2(CYS27 - PRO220) including a C-terminal His tag was expressed in HEK293 cells.
Molecular weight
Recombinant human TIMP2 is a protein consisting of 200 amino acid residue subunits,due to glycosylation migrates as an approximately as 22kDa band protein on reduced SDS-PAGE.
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological activity
The activity was tested by the ability of recombinant human TIMP2 to inhibit human MMP?2 cleavage of a fluorogenic peptide substrate, the calculated ED50 is 4nM.
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human TIMP-2 is lyophilized from 0.2 μm filtered PBS solution, pH7.2 , 5% Trehalose.
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage
Recombinant TIMP2 can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to-70°Cfor twelve months. Avoid repeated freeze/thaw cycles.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Molecular function
Molecular function
