Optimized DNA sequence encoding Human extracellular domain of human Neprilysin fused to a N -term polyHis tag was expressed in HEK293 cells.
Recombinant CD10 is a monomer protein consisting of 718 amino acid residue subunits, due to glycosylation migrates as an approximately100 kDa protein on SDS-PAGE.
>95%, as determined by SDS-PAGE and HPLC
Measured by its ability to cleave flourogenic peptide substrate Mca-RPPGFSAFK(Dnp)-OH. The specific activity was measured to be >1nMole/min/ug
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Recombinant CD10 is supplied as a 0.2 μm filtered solution in 20mM Tris,100mM NaCL, pH8.0 with 5% Trehalose
To prepare stock, reconstitute by gently mixing in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.DO NOT VORTEX
Recombinant Neprilysin, as supplied, can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with 0.1% HSA as carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.