Optimized DNA sequence encoding extracellular domain of Mouse lysosomal integral membrane protein (LIMP-II) (ARG27 - THR432) including C-terminal His tag was expressed in HEK293 cells.
Recombinant LIMP-II is a protein consisting of 415 amino acid residue subunits, due to glycosylation migrates as an approximately 70kDa protein on reduced SDS-PAGE.
>95%, as determined by SDS-PAGE and HPLC
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Recombinant Mouse LIMP-2 (LIMP-II) is lyophilized from 0.2 μm filtered PBS solution, pH7.2 , 5% Trehalose.
Recombinant SR-BI can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to-70°Cfor twelve months. Avoid repeated freeze/thaw cycles.
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.