Biotinylated Mouse IL-2 Protein Recombinant
Categories: Biotinylated cytokinesBiotinylated proteins$542.00 – $1,805.00
Description
Accession
P04351
Source
DNA sequence encoding Mouse IL-2 including a N-His-Avi tag was expressed in HEK293 Cells.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
Molecular weight
Recombinant Mouse IL-2 is a protein consisting of amino acid residues [Ala21-Gln169] ,The protein has a predicted MW of 20.1 kDa. Due to glycosylation, the protein migrates to 27-30 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Mouse IL-2 was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Biological Process
Molecular function
Molecular function
Methods
Biotinylated Mouse IL-2 Protein Tris-Bis PAGE
- Biotinylated Mouse IL-2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Biotinylated Mouse IL-2 Protein SEC-HPLC
- The purity of Biotinylated Mouse IL-2 is greater than 95% as determined by SEC-HPLC.
Biotinylated Mouse IL-2 Protein ELISA Data
- Immobilized Mouse IL-2 R alpha, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Mouse IL-2, His Tag with the EC50 of 95.8ng/ml determined by ELISA.
