Biotinylated Human Siglec-2/CD22 Protein Recombinant
Category: Biotinylated proteins$542.00 – $1,805.00
Description
Accession
P20273
Source
DNA sequence encoding Human Siglec-2/CD22 including a C-His-Avi tag was expressed in HEK293 Cells.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
Molecular weight
Recombinant Human Siglec-2/CD22 is a protein consisting of amino acid residues [Asp20-Arg687] ,The protein has a predicted MW of 78.1 kDa. Due to glycosylation, the protein migrates to 110-120 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human Siglec-2/CD22 was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Biological Process
Methods
Biotinylated Human Siglec-2/CD22 Protein Tris-Bis PAGE
- Biotinylated Human Siglec-2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%..
Biotinylated Human Siglec-2/CD22 Protein SEC-HPLC
- The purity of Biotinylated Human Siglec-2 is greater than 95% as determined by SEC-HPLC.
Biotinylated Human Siglec-2/CD22 Protein ELISA Data
- Immobilized Anti-Siglec-2 Antibody, hFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human Siglec-2, His Tag with the EC50 of 19.1ng/ml determined by ELISA.
