Biotinylated Human NKp30/NCR3/CD337 Protein Recombinant
Category: Biotinylated proteins$542.00 – $1,805.00
Description
Accession
AAH52582
Source
DNA sequence encoding Human NKp30/NCR3/CD337 including a C-His-Avi tag was expressed in HEK293 Cells.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
Molecular weight
Recombinant Human NKp30/NCR3/CD337 is a protein consisting of amino acid residues [Leu19-Thr138] ,The protein has a predicted MW of 20 kDa. Due to glycosylation, the protein migrates to 26-45 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human NKp30/NCR3/CD337 was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Methods
Biotinylated Human NKp30/NCR3/CD337 Protein Tris-Bis PAGE
- Biotinylated Human NKp30 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Biotinylated Human NKp30/NCR3/CD337 Protein SEC-HPLC
- The purity of Biotinylated Human NKp30 is greater than 95% as determined by SEC-HPLC.
Biotinylated Human NKp30/NCR3/CD337 Protein ELISA Data
- Immobilized Anti-NKp30 Antibody, hFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human NKp30, His Tag with the EC50 of 30ng/ml determined by ELISA.
Biotinylated Human NKp30/NCR3/CD337 Protein SPR Data
- Human B7-H6, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human NKp30, His-Avi Tag with an affinity constant of 0.330 μM as determined in SPR assay (Biacore T200).
