Biotinylated Human LAP (TGF beta 1) Protein Recombinant
Category: Biotinylated proteins$618.00 – $2,062.00
Description
Accession
P01137
Source
DNA sequence encoding Human LAP (TGF beta 1) including a N-His-Avi tag was expressed in HEK293 Cells.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
Molecular weight
Recombinant Human LAP (TGF beta 1) is a protein consisting of amino acid residues [Leu30-Arg278(C33S)] ,The protein has a predicted MW of 31.4 kDa. Due to glycosylation, the protein migrates to 45-50 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human LAP (TGF beta 1) was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Interactor
Interactor
Interactor
P18564
Interactor
Interactor
Interactor
Q03167
Interactor
Interactor
Q14766
Interactor
Molecular function
Molecular function
Methods
Biotinylated Human LAP (TGF beta 1) Protein Tris-Bis PAGE
- Biotinylated Human LAP (TGF beta 1) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Biotinylated Human LAP (TGF beta 1) Protein SEC-HPLC
- The purity of Biotinylated Human LAP (TGF beta 1) is greater than 95% as determined by SEC-HPLC.
Biotinylated Human LAP (TGF beta 1) Protein ELISA Data
- Immobilized Biotinylated Human LAP (TGF beta 1) , His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Human TGF-beta RII, hFc Tag with the EC50 of 23.8ng/ml determined by ELISA.
Biotinylated Human LAP (TGF beta 1) Protein ELISA Data
- Immobilized Human ITGAV&ITGB6, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human LAP (TGF beta 1) , His Tag with the EC50 of 0.86μg/ml determined by ELISA.
