Biotinylated Human IL-18 R1/CD218a Protein Recombinant
Category: Biotinylated proteins$542.00 – $1,805.00
Description
Accession
AAH69575
Source
DNA sequence encoding Human IL-18 R1/CD218a including a C-His-Avi tag was expressed in HEK293 Cells.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
Molecular weight
Recombinant Human IL-18 R1/CD218a is a protein consisting of amino acid residues [Ala19-Arg329] ,The protein has a predicted MW of 38.8 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human IL-18 R1/CD218a was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Methods
Biotinylated Human IL-18 R1/CD218a Protein Tris-Bis PAGE
- Biotinylated Human IL-18 R1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Biotinylated Human IL-18 R1/CD218a Protein SEC-HPLC
- The purity of Biotinylated Human IL-18 R1 is greater than 95% as determined by SEC-HPLC.
Biotinylated Human IL-18 R1/CD218a Protein SPR Data
- Biotinylated Human IL-18 R1, His Tag captured on CM5-SA Chip can bind Human IL-18, No Tag with an affinity constant of 1.4nM as determined in SPR assay (Biacore T200).
