Biotinylated Human Her3/ErbB3 Protein Recombinant
Category: Biotinylated proteins$542.00 – $1,805.00
Description
Accession
P21860
Source
DNA sequence encoding Human Her3/ErbB3 including a C-His-Avi tag was expressed in HEK293 Cells.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
Molecular weight
Recombinant Human Her3/ErbB3 is a protein consisting of amino acid residues [Ser20-Thr643] ,The protein has a predicted MW of 71.6 kDa. Due to glycosylation, the protein migrates to 90-100 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human Her3/ErbB3 was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Interactor
Interactor
Interactor
O41931
Interactor
Interactor
Interactor
P27986
Interactor
Interactor
Molecular function
Molecular function
Methods
Biotinylated Human Her3/ErbB3 Protein Tris-Bis PAGE
- Biotinylated Human Her3 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Biotinylated Human Her3/ErbB3 Protein SEC-HPLC
- The purity of Biotinylated Human Her3 is greater than 95% as determined by SEC-HPLC.
Biotinylated Human Her3/ErbB3 Protein ELISA Data
- Immobilized Anti-Her3 Antibody, hFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylatd Human Her3, His Tag with the EC50 of 0.20μg/ml determined by ELISA.
