Biotinylated Human GM-CSF R alpha Protein Recombinant
Category: Biotinylated proteins$542.00 – $1,805.00
Description
Accession
P15509
Source
DNA sequence encoding Human GM-CSF R alpha including a C-His-Avi tag was expressed in HEK293 Cells.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
Molecular weight
Recombinant Human GM-CSF R alpha is a protein consisting of amino acid residues [Glu23-Gly320] ,The protein has a predicted MW of 37.7 kDa. Due to glycosylation, the protein migrates to 68-75 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human GM-CSF R alpha was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Interactor
Molecular function
Methods
Biotinylated Human GM-CSF R alpha Protein Tris-Bis PAGE
- Biotinylated Human GM-CSF R alpha on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%.
Biotinylated Human GM-CSF R alpha Protein SEC-HPLC
- The purity of Biotinylated Human GM-CSF R alpha is greater than 95% as determined by SEC-HPLC.
Biotinylated Human GM-CSF R alpha Protein ELISA Data
- Immobilized Anti-GM-CSF R alpha Antibody, hFc Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human GM-CSF R alpha, His Tag with the EC50 of 11.3ng/ml determined by ELISA.
Biotinylated Human GM-CSF R alpha Protein SPR Data
- Biotinylated Human GM-CSF R alpha, His-Avi Tag captured on CM5 Chip via Anti-His Antibody can bind Human GM-CSF, No Tag with an affinity constant of 1.68 nM as determined in SPR assay (Biacore T200).
