Biotinylated Human Fc gamma RIIA/CD32a (H167) Protein Recombinant
Category: Biotinylated proteins$542.00 – $1,805.00
Description
Accession
P12318
Source
DNA sequence encoding Human Fc gamma RIIA/CD32a (H167) including a C-His-Avi tag was expressed in HEK293 Cells.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
Molecular weight
Recombinant Human Fc gamma RIIA/CD32a (H167) is a protein consisting of amino acid residues [Ala36-Ile218(H167)] ,The protein has a predicted MW of 23.2 kDa. Due to glycosylation, the protein migrates to 30-40 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human Fc gamma RIIA/CD32a (H167) was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Biological Process
Molecular function
Molecular function
Methods
Biotinylated Human Fc gamma RIIA/CD32a (H167) Protein Tris-Bis PAGE
- Human Fc gamma RIIA/CD32a (H167) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Biotinylated Human Fc gamma RIIA/CD32a (H167) Protein SEC-HPLC
- The purity of Human Fc gamma RIIA/CD32a (H167) is greater than 95% as determined by SEC-HPLC.
Biotinylated Human Fc gamma RIIA/CD32a (H167) Protein SPR Data
- Rituximab captured on CM5 Chip via Protein A can bind Biotinylated Human Fc gamma RIIA (H167) , His-Avi Tag with an affinity constant of 0.680 μM as determined in SPR assay (Biacore T200).
