Biotinylated Human EGFRVIII Protein Recombinant
Category: Biotinylated proteins$542.00 – $1,805.00
Description
Accession
NP_001333870
Source
DNA sequence encoding Human EGFRVIII including a C-His-Avi tag was expressed in HEK293 Cells.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
Molecular weight
Recombinant Human EGFRVIII is a protein consisting of amino acid residues [Leu25-Ser378] ,The protein has a predicted MW of 41.6 kDa. Due to glycosylation, the protein migrates to 65-78 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human EGFRVIII was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Methods
Biotinylated Human EGFRVIII Protein Tris-Bis PAGE
- Biotinylated Human EGFRVIII on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Biotinylated Human EGFRVIII Protein SEC-HPLC
- The purity of Biotinylated Human EGFRVIII is greater than 95% as determined by SEC-HPLC.
Biotinylated Human EGFRVIII Protein ELISA Data
- Immobilized Biotinylated Human EGFRVIII, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-EGFRVIII Antibody, hFc Tag with the EC50 of 16.1ng/ml determined by ELISA.
