Biotinylated Human E-Selectin/CD62E Protein Recombinant
Category: Biotinylated proteins$542.00 – $1,805.00
Description
Accession
P16581
Source
DNA sequence encoding Human E-Selectin/CD62E including a C-His-Avi tag was expressed in HEK293 Cells.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
Molecular weight
Recombinant Human E-Selectin/CD62E is a protein consisting of amino acid residues [Trp22-Pro556] ,The protein has a predicted MW of 61.6 kDa. Due to glycosylation, the protein migrates to 80-100 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human E-Selectin/CD62E was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Interactor
O00592
Interactor
Interactor
Interactor
Biological Process
Methods
Biotinylated Human E-Selectin/CD62E Protein Tris-Bis PAGE
- Biotinylated Human E-selectin on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Biotinylated Human E-Selectin/CD62E Protein SEC-HPLC
- The purity of Biotinylated Human E-selectin is greater than 95% as determined by SEC-HPLC.
Biotinylated Human E-Selectin/CD62E Protein ELISA Data
- Immobilized Biotinylated Human E-Selectin at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-E-Selectin Antibody, hFc Tag with the EC50 of 7.8ng/ml determined by ELISA.
