Biotinylated Human CTGF/CCN2 Protein Recombinant
Category: Biotinylated proteins$542.00 – $1,805.00
Description
Accession
Q5M8T4
Source
DNA sequence encoding Human CTGF/CCN2 including a C-His-Avi tag was expressed in HEK293 Cells.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
Molecular weight
Recombinant Human CTGF/CCN2 is a protein consisting of amino acid residues [Gln27-Ala349] ,The protein has a predicted MW of 38.4 kDa. Due to glycosylation, the protein migrates to 43-53 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human CTGF/CCN2 was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Methods
Biotinylated Human CTGF/CCN2 Protein Tris-Bis PAGE
- Biotinylated Human CTGF on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Biotinylated Human CTGF/CCN2 Protein ELISA Data
- Immobilized Anti-CTGF Antibody at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human CTGF, His Tag with the EC50 of 32.6ng/ml determined by ELISA.
