//Biotinylated Human CD3E&CD3D/CD3 epsilon&CD3 delta Protein Recombinant

Biotinylated Human CD3E&CD3D/CD3 epsilon&CD3 delta Protein Recombinant

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$618.00$2,062.00

SKU: KTWY5R9FB

Description

Accession
P07766
Source
DNA sequence encoding Human CD3E&CD3D/CD3 epsilon&CD3 delta including a C-hFc-Avi tag was expressed in HEK293 Cells.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
Molecular weight
Recombinant Human CD3E&CD3D/CD3 epsilon&CD3 delta is a protein consisting of amino acid residues [Asp23-Asp126(CD3E) & Phe22-Ala105(CD3D)] ,The protein has a predicted MW of 40 kDa (CD3E) and 35.4 kDa (CD3D). Due to glycosylation,the protein migrates to 48-60 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human CD3E&CD3D/CD3 epsilon&CD3 delta was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Biological Process
Molecular function

Methods

Biotinylated Human CD3E&CD3D/CD3 epsilon&CD3 delta Protein Tris-Bis PAGE

  • Biotinylated Human CD3E&CD3D on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Biotinylated Human CD3E&CD3D/CD3 epsilon&CD3 delta Protein SEC-HPLC

  • The purity of Biotinylated Human CD3E&CD3D was greater than 95% as determined by SEC-HPLC.

Biotinylated Human CD3E&CD3D/CD3 epsilon&CD3 delta Protein ELISA Data

  • Immobilized Biotinylated Human CD3E&CD3D, hFc Tag at 1μg/ml (100μl/Well) on streptavidin (5μg/ml) precoated plate. Dose response curve for OKT3, mFc Tag with the EC50 of 27.7ng/ml determined by ELISA.
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