Biotinylated Human CD3E&CD3D/CD3 epsilon&CD3 delta Protein Recombinant
Category: Biotinylated proteins$618.00 – $2,062.00
Description
Accession
P07766
Source
DNA sequence encoding Human CD3E&CD3D/CD3 epsilon&CD3 delta including a C-hFc-Avi tag was expressed in HEK293 Cells.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
Molecular weight
Recombinant Human CD3E&CD3D/CD3 epsilon&CD3 delta is a protein consisting of amino acid residues [Asp23-Asp126(CD3E) & Phe22-Ala105(CD3D)] ,The protein has a predicted MW of 40 kDa (CD3E) and 35.4 kDa (CD3D). Due to glycosylation,the protein migrates to 48-60 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human CD3E&CD3D/CD3 epsilon&CD3 delta was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Biological Process
Molecular function
Methods
Biotinylated Human CD3E&CD3D/CD3 epsilon&CD3 delta Protein Tris-Bis PAGE
- Biotinylated Human CD3E&CD3D on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Biotinylated Human CD3E&CD3D/CD3 epsilon&CD3 delta Protein SEC-HPLC
- The purity of Biotinylated Human CD3E&CD3D was greater than 95% as determined by SEC-HPLC.
Biotinylated Human CD3E&CD3D/CD3 epsilon&CD3 delta Protein ELISA Data
- Immobilized Biotinylated Human CD3E&CD3D, hFc Tag at 1μg/ml (100μl/Well) on streptavidin (5μg/ml) precoated plate. Dose response curve for OKT3, mFc Tag with the EC50 of 27.7ng/ml determined by ELISA.
