Biotinylated Human B7-2/CD86 Protein Recombinant
Category: Biotinylated proteins$542.00 – $1,805.00
Description
Accession
P42081
Source
DNA sequence encoding Human B7-2/CD86 including a C-His-Avi tag was expressed in HEK293 Cells.
The recombinant protein was then test biotinylated site specific using the AVItag biotinylation technology.test
The recombinant protein was then test biotinylated site specific using the AVItag biotinylation technology.test
Molecular weight
Recombinant Human B7-2/CD86 is a protein consisting of amino acid residues [Leu26-Pro247] ,The protein has a predicted MW of 28.2 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human B7-2/CD86 was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Interactor
Biological Process
Biological Process
Molecular function
Methods
Biotinylated Human B7-2/CD86 Protein Tris-Bis PAGE
- Biotinylated Human B7-2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Biotinylated Human B7-2/CD86 Protein ELISA Data
- Immobilized Biotinylated Human B7-2, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Human CTLA-4, hFc Tag with the EC50 of 0.2μg/ml determined by ELISA.
