Biotinylated Human ACE2/ACEH Protein Recombinant
Category: Biotinylated proteins$542.00 – $1,805.00
Description
Accession
Q9BYF1
Source
DNA sequence encoding Human ACE2/ACEH including a C-His-Avi tag was expressed in HEK293 Cells.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
Molecular weight
Recombinant Human ACE2/ACEH is a protein consisting of amino acid residues [Gln18-Ser740] ,The protein has a predicted MW of 86.5 kDa. Due to glycosylation, the protein migrates to 95-110 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human ACE2/ACEH was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Interactor
Interactor
Q6Q1S2
Biological Process
Molecular function
Molecular function
Methods
Biotinylated Human ACE2/ACEH Protein Tris-Bis PAGE
- Biotinylated Human ACE2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Biotinylated Human ACE2/ACEH Protein SEC-HPLC
- The purity of Biotinlylated human ACE2 is greater than 95% as determined by SEC-HPLC.
Biotinylated Human ACE2/ACEH Protein ELISA Data
- Immobilized SARS-COV-2 S1, His Tag at 2μg/ml (100μl/Well). Dose response curve for Biotinylated Human ACE2, His Tag with EC50 of 0.24μg/ml determined by ELISA.
Biotinylated Human ACE2/ACEH Protein
- Immobilized SARS-COV-2 Spike RBD, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human ACE2, His Tag with the EC50 of 0.06μg/ml determined by ELISA.
