Biotinylated Cynomolgus CD3E&CD3D/CD3 epsilon&CD3 delta Protein Recombinant
Category: Biotinylated proteins$542.00 – $1,805.00
Description
Accession
Q95LI5
Source
DNA sequence encoding Cynomolgus CD3E&CD3D/CD3 epsilon&CD3 delta (Primary Amine Labeling) including a C-hFc tag was expressed in HEK293 Cells.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
Molecular weight
Recombinant Cynomolgus CD3E&CD3D/CD3 epsilon&CD3 delta (Primary Amine Labeling) is a protein consisting of amino acid residues [Gln22-Asp117(CD3E) & Phe22-Ala105(CD3D)] ,The protein has a predicted MW of 36.9 kDa (CD3E) and 35.4 kDa (CD3D). Due to glycosylation,the protein migrates to 58-62 kDa and 44-46 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Cynomolgus CD3E&CD3D/CD3 epsilon&CD3 delta (Primary Amine Labeling) was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Methods
Biotinylated Cynomolgus CD3E&CD3D/CD3 epsilon&CD3 delta Protein (Primary Amine Labeling) Tris-Bis PAGE
- Biotinylated Cynomolgus CD3E&CD3D on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%.
Biotinylated Cynomolgus CD3E&CD3D/CD3 epsilon&CD3 delta Protein (Primary Amine Labeling) SEC-HPLC
- The purity of Biotinylated Cynomolgus CD3E&CD3D was greater than 95% as determined by SEC-HPLC.
Biotinylated Cynomolgus CD3E&CD3D/CD3 epsilon&CD3 delta Protein (Primary Amine Labeling) ELISA Data
- Immobilized Anti-CD3, hFc Tag Antibody at 5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Cynomolgus CD3E&CD3D, hFc Tag with the EC50 of 58.2ng/ml determined by ELISA.
