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Human RANTES-CCL5 Recombinant

RANTES

accession:  P13501
   Size A     5ug   $ 70
   Size B      20ug  $160
   Size C      1mg  $ 2700
Domain  :  IL8
Gene  :  CCL5
Catalog no. :  RKP13501
Source:
Optimized DNA sequence encoding Human Rantes/CCL5 mature chain was expressed in Escherichia Coli.

 
Molecular weight:

Native human RANTES/CCL5 is generated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately 8 kDa.

Recombinant RANTES/CCL5 is a disulfide-linked monomer protein consisting of 69 amino acid residue subunits and migrates as an approximately 8 kDa protein under non-reducing and reducing conditions in SDS-PAGE.


 
Purity:
>98%, as determined by SDS-PAGE and HPLC

 
Biological Activity:
Determined by its ability to chemoattract human blood monocytes using a concentration range of 1.0-10.0 ng/ml.

 
Protein Sequence:
        10         20         30         40         50         60 
MKVSAAALAV ILIATALCAP ASASPYSSDT TPCCFAYIAR PLPRAHIKEY FYTSGKCSNP 

        70         80         90 
AVVFVTRKNR QVCANPEKKW VREYINSLEM S 
(*)Complete precursor sequence shown, expressed chain highlighted
 
Endotoxin:
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

 
Presentation:
Recombinant Rantes/CCL5 was lyophilized from a 0.2 μm filtered PBS solution.

 
Reconstitution:
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

 
Storage:
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity.

Avoid repeated freeze/thaw cycles.

 
Usage:
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

 

RANTES

RANTES belongs to the family of chemotactic cytokines known as Chemokines. RANTES is expressed by an early response gene. The synthesis of RANTES is induced by TNF-alpha and IL1-alpha.RANTES is chemotactic for T-cells, human eosinophils and basophils and plays an active role in recruiting leukocytes into inflammatory sites. RANTES also activates eosinophils to release, for example, eosinophilic cationic protein. It changes the density of eosinophils and makes them hypodense, which is thought to represent a state of generalized cell activation and is associated most often with diseases such as asthma and allergic rhinitis. RANTES also is a potent activator of oxidative metabolism specific for eosinophils. RANTES increases the adherence of monocytes to endothelial cells. It selectively supports the migration of monocytes and T-lymphocytes expressing the cell surface markers CD4 and UCHL1. These cells are thought to be pre-stimulated T-helper cells with memory T-cell functions. RANTES activates human basophils from some select basophil donors and causes the release of histamines.

Related Publications:
withdrawn: very strong synergy between modified RANTES and gp41 binding peptides leads to potent anti-hiv-1 activity
Antimicrob. Agents Chemother., Aug 2009; 10.1128/AAC.01250-07.
8-prenylkaempferol suppresses influenza a virus-induced RANTES production in a549 cells via blocking pi3k-mediated transcriptional activation of nf-b and irf3
Evid. Based Complement. Altern. Med., Jul 2009; 10.1093/ecam/nep066.
RANTES release by human adipose tissue in vivo and evidence for depot-specific differences
Am J Physiol Endocrinol Metab, Jun 2009; 296: E1262 - E1268.
engineering the glycosaminoglycan-binding affinity, kinetics and oligomerization behavior of RANTES: a tool for generating chemokine-based glycosaminoglycan antagonists
Protein Eng. Des. Sel., Jun 2009; 22: 367 - 373.
interaction between RANTES promoter variant and ccr532 favors recovery from hepatitis b
J. Immunol., Dec 2008; 181: 7944 - 7947.




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product_icon_8.jpg Human RANTES-CCL5 Recombinant
 
 
 
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