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Human Interleukin-1 receptor antagonist Recombinant

IL-1

accession:  P18510
   Size A     20ug   $ 70
   Size B      100ug  $160
   Size C      1mg  $ 900
Domain  :  Interleukin-1
Gene  :  IL1RA
Catalog no. :  RKP18510
Source:
Optimized DNA sequence encoding Human interleukin-1 receptor antagonist mature chain was expressed in Escherichia Coli.

 
Molecular weight:

Human interleukin-1 receptor antagonist is generated by the proteolytic removal of the signal peptide and propeptide.The molecule has a calculated molecular mass of approximately 17kDa.

Recombinant IL-1RA is a disulfide-linked monomer protein consisting of 153 amino acid residue subunits, and migrates as an approximately 17 kDa protein under non-reducing and reducing conditions in SDS-PAGE.


 
Purity:
>95%, as determined by SDS-PAGE and HPLC

 
Biological Activity:
The ED(50) was determined by the ability to inhibit the IL-1α mediated cell proliferation in a murine helper T cell line,for this effect, in the presence of 50 pg/mL of IL-1α, is typically 20 - 60 ng/mL.

 
Protein Sequence:
        10         20         30         40         50         60 
MEICRGLRSH LITLLLFLFH SETICRPSGR KSSKMQAFRI WDVNQKTFYL RNNQLVAGYL 

        70         80         90        100        110        120 
QGPNVNLEEK IDVVPIEPHA LFLGIHGGKM CLSCVKSGDE TRLQLEAVNI TDLSENRKQD 

       130        140        150        160        170 
KRFAFIRSDS GPTTSFESAA CPGWFLCTAM EADQPVSLTN MPDEGVMVTK FYFQEDE 
(*)Complete precursor sequence shown, expressed chain highlighted
 
Endotoxin:
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

 
Presentation:
Recombinant Interleukin-1 receptor antagonist was lyophilized from a 0.2 μm filtered  PBS solution pH 7.4.

 
Reconstitution:
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

 
Storage:
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity.

Avoid repeated freeze/thaw cycles.

 
Usage:
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

 

 

interleukin-1 receptor antagonist

IL1ra is found in the conditioned medium of a variety of cells and is produced by macrophages, monocytes,neutrophils. The soluble form of IL1ra has been shown to be produced by hepatocytes, and to be regulated by pro- inflammatory cytokines.

Related Publications:
9e,11e-conjugated linoleic acid increases expression of the endogenous antiinflammatory factor, interleukin-1 receptor antagonist, in raw 264.7 cells
J. Nutr., Oct 2009; 139: 1861 - 1866.
sustained effects of interleukin-1 receptor antagonist treatment in type 2 diabetes
Diabetes Care, Sep 2009; 32: 1663 - 1668.
an autoinflammatory disease with deficiency of the interleukin-1–receptor antagonist
N. Engl. J. Med., Jun 2009; 360: 2426 - 2437.
accelerated recovery from acute hypoxia in obese mice is due to obesity-associated up-regulation of interleukin-1 receptor antagonist
Endocrinology, Jun 2009; 150: 2660 - 2667.
phosphate buffered saline containing calcium and magnesium elicits increased secretion of interleukin-1 receptor antagonist
Lab Med, May 2009; 40: 290 - 293.




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Antibodies:
Purified Polyclonal
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