Optimized DNA sequence encoding Mouse SCF mature chain was expressed in E.Coli
Native Mouse Stem Cell Factor is generated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately 18kDa. Recombinant SCF is a monomer protein consisting of 165 amino acid residue subunits, and due to glycosylation migrates as an approximately 18 kDa protein under non-reducingand reducing conditions in SDS-PAGE.
>95%, as determined by SDS-PAGE and HPLC
The ED(50) was determined by the dose-dependent stimulation of theproliferation of human TF-1 cells is ≤.0 ng/ml, corresponding to a specific activity of ≥ x units/mg.
MKKTQTWIIT CIYLQLLLFN PLVKTKEICG NPVTDNVKDI TKLVANLPND YMITLNYVAG MDVLPSHCWL RDMVIQLSLS LTTLLDKFSN ISEGLSNYSI IDKLGKIVDD LVLCMEENAP KNIKESPKRP ETRSFTPEEF FSIFNRSIDA FKDFMVASDT SDCVLSSTLG PEKDSRVSVT KPFMLPPVA A SSLRNDSSSS NRKAAKAPED SGLQWTAMAL PALISLVIGF AFGALYWKKK QSSLTRAVEN IQINEEDNEI SMLQQKEREF QEV
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg).
Recombinant Stem cell factor was lyophilized from a.2 μm filtered solution in PBS, pH.4.
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers.
The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at° -° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.