DNA sequence encoding the mature chain of Mouse IFNA2 was expressed in Insect Cells.
Recombinant Mouse IFNA2 is a protein consisting of amino acid residues [Cys24-Glu190] ,due to glycosylation migrates as an approximately 31 kDa protein on SDS-PAGE.
>95%, as determined by SDS-PAGE and HPLC
The activity was determined by a viral resistance assay of Human WISH cells, and was found to be in the range of 1x10^8 IU/mg.
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Recombinant IFNA2 was lyophilized from 0.2 µm filtered PBS solution pH7.4
MARLCAFLVM LIVMSYWSIC SLGCDLPHTY NLRNKRALKV LAQMRRLPFL SCLKDRQDFG FPLEKVDNQQ IQKAQAIPVL RDLTQQTLNL FTSKASSAAW NATLLDSFCN DLHQQLNDLQ TCLMQQVGVQ EPPLTQEDAL LAVRKYFHRI TVYLREKKHS PCAWEVVRAE VWRALSSSVN LLPRLSEEKE
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.