Human LIN28 Recombinant
Categories: CytokinesHuman reagentsRecombinant Human Cytokines$70.00 – $3,500.00
Description
Accession
Q9H9Z2
Source
DNA sequence encoding mature chain of Human LIN28 was expressed in E.coli cells.
Molecular weight
Recombinant Human LIN8 is a monomer consisting of 208 amino acid residue subunits and migrates as an approximately 25 kDa protein under reducing conditions in SDS-PAGE.
Purity
>95%, as determined by SDS-PAGE
Biological Activity
The activity was determined by its ability to bind recombinant Human with a linear range of 60-1000 ng/ml in functional ELISA .
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human LIN28 was lyophilized from a 0.2 μm filtered PBS solution pH 7.4.
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
