Optimized DNA sequence encoding extracellular domain of Mouse CD40 including a C-terminal His tag was expressed in HEK293 cells.
Recombinant CD40 is a protein consisting of 180 amino acid residue subunits, due to glycosylation migrates as an approximately 30kDa protein on SDS-PAGE.
>97%, as determined by SDS-PAGE and HPLC
The activity was tested by the ability of immobilized recombinant mouse CD40 to bind Human CD40 Ligand with a linear range of 10-500 ng/ml
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Recombinant Mouse CD40 is lyophilized from 0.2 μm filtered PBS solution, pH7.2 , 5% Trehalose.
Recombinant CD40 can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to-70°Cfor twelve months. Avoid repeated freeze/thaw cycles.
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.