Human VEGF 121AA Recombinant (E.Coli)

$70.00 – $3,500.00

SKU: RKP156921 Tags: P15692, vegf, VEGF-A, VEGFA, VPF

Description

Accession

P15692

Source

Optimized DNA sequence encoding Human Vascular Endothelial Growth Factor 121AA mature chain was expressed in Escherichia Coli.

Molecular weight

Native human VEGF 121AA is generated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately 20kDa. Recombinant Vascular endothelial growth factor is a disulfide-linked homodimer protein consisting of 2x121 amino acid residue subunits, and migrates as an approximately 40kDa protein under non-reducing and as 20kDa under reducing conditions in SDS-PAGE.

Purity

>95%, as determined by SDS-PAGE and HPLC

Biological Activity

The ED(50) was determined by the dose-dependent proliferation ofhuman umbilical vein endothelial cells and was found to be in the range of.0-4.0 ng/ml.

Protein Sequence

MNFLLSWVHW SLALLLYLHH AKWSQAAPMA EGGGQNHHEV VKFMDVYQRS YCHPIETLVD IFQEYPDEIE YIFKPSCVPL MRCGGCCNDE GLECVPTEES NITMQIMRIK PHQGQHIGEM SFLQHNKCEC RPKKDRARQE NCDKPRR

Endotoxin

Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

Presentation

Recombinant VEGFwas lyophilized from.2 μm filtered PBS solution, pH7.0.

Reconstitution

A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

Storage

The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at2° -8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

Usage

This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

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