DNA sequence encoding extracellular domain of Human LYN to a N-terminal GST tag was expressed in Insect cells.
Recombinant Human LYN is a monomer consisting of 736 amino acid residue , and migrates as an approximately 75 kDa protein under reducing conditions in SDS-PAGE.
>95%, as determined by SDS-PAGE
The activity was determined using a Glu/Tyr peptide substrate in a fluorogenic assay , and was determined to be 100nM/min/mg.
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Recombinant Human LYN was lyophilized from a 0.2 μm filtered 20mM Tris,50mM NaCl, pH 8.0, 0.5mM PMSF.
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.