Optimized DNA sequence encoding Human Cystatin D-CST5(GLY21 - VAL142 ) mature chain including a C-terminal His tag was expressed in HEK293 cells.
Recombinant Human Cystatin D (CST5) is a protein consisting of 127 amino acid residue subunits,due to glycosylation migrates as an approximately as 17kDa band protein on reduced SDS-PAGE.
>95%, as determined by SDS-PAGE and HPLC
The activity was tested by the ability to inhibit papain cleavage of a fluorogenic substrate. The ED50 was measured to < 15nM
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Recombinant Cystatin 5 is lyophilized from 0.2 μm filtered PBS solution, pH7.2 , 5% Trehalose.
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Recombinant Human Cystatin D (CST5) can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to-70°Cfor twelve months. Avoid repeated freeze/thaw cycles.
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.