Human 4-1BB (CD137) Recombinant
Category: Surface & Soluble Receptors$160.00 – $1,950.00
Description
Accession
Q07011
Source
Optimized DNA sequence encoding Human 4-1BB (CD137) fused to a C terminal IgG1 Fc tag was expressed in CHO Cells.
Molecular weight
Recombinant Human 4-1BB (CD137) is a homodimer protein consisting of two 410 amino acid residue subunits, due to glycosylation migrates as an approximately 65kDa protein on SDS-PAGE under reducing conditions.
Purity
>97%, as determined by SDS-PAGE and HPLC
Biological Activity
The activity was tested by the ability of immobilized recombinant mouse 4-1BBL to bind human 4-1BB with a linear range of 10-500 ng/ml
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant mouse CD137 is lyophilized from 0.2 μm filtered PBS solution, pH7.2 .
Storage
Recombinant Mouse 4-1BB can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to-70°Cfor twelve months. Avoid repeated freeze/thaw cycles.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Interactor
Molecular function
Methods
CD137L signaling activates microglia.
- Microglia cells were grown on plates that had been coated with PBS (grey bars) or 10 μg/mL of Fc control protein (white bars) or 10 μg/mL of CD137-Fc protein (black bars).
