Biotinylated Human VEGF R2/KDR Protein Recombinant
Category: Biotinylated proteins$542.00 – $1,805.00
Description
Accession
P35968
Source
DNA sequence encoding Human VEGF R2/KDR including a C-His-Avi tag was expressed in HEK293 Cells.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
Molecular weight
Recombinant Human VEGF R2/KDR is a protein consisting of amino acid residues [Ala20-Glu764] ,The protein has a predicted MW of 86.2 kDa. Due to glycosylation, the protein migrates to 115-140 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human VEGF R2/KDR was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Interactor
Interactor
Interactor
Interactor
Interactor
P52584
Interactor
Interactor
Biological Process
Biological Process
Biological Process
Molecular function
Molecular function
Molecular function
Methods
Biotinylated Human VEGF R2/KDR Protein Tris-Bis PAGE
- Biotinylated Human VEGF R2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Biotinylated Human VEGF R2/KDR Protein SEC-HPLC
- The purity of Biotinylated Human VEGF R2 is greater than 95% as determined by SEC-HPLC.
Biotinylated Human VEGF R2/KDR Protein ELISA Data
- Immobilized Anti-VEGF R2 Antibody, hFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human VEGF R2, His Tag with the EC50 of 0.35μg/ml determined by ELISA.