Biotinylated Human SIRP Beta/CD172b Protein Recombinant
Category: Biotinylated proteins$542.00 – $1,805.00
Description
Accession
O00241
Source
DNA sequence encoding Human SIRP Beta/CD172b including a C-His-Avi tag was expressed in HEK293 Cells.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
Molecular weight
Recombinant Human SIRP Beta/CD172b is a protein consisting of amino acid residues [Glu30-Ala369] ,The protein has a predicted MW of 40 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human SIRP Beta/CD172b was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Methods
Biotinylated Human SIRP Beta/CD172b Protein Tris-Bis PAGE
- Biotinylated Human SIRP Beta on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Biotinylated Human SIRP Beta/CD172b Protein SEC-HPLC
- The purity of Biotinylated Human SIRP Beta is greater than 95% as determined by SEC-HPLC.
