Biotinylated Human SIRP alpha V2/CD172a Protein Recombinant
Category: Biotinylated proteins$542.00 – $1,805.00
Description
Accession
AAH26692
Source
DNA sequence encoding Human SIRP alpha V2/CD172a including a C-His-Avi tag was expressed in HEK293 Cells.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
Molecular weight
Recombinant Human SIRP alpha V2/CD172a is a protein consisting of amino acid residues [Glu31-Arg369] ,The protein has a predicted MW of 40 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/Āµg(1EU/Āµg).
Presentation
Recombinant Human SIRP alpha V2/CD172a was lyophilized from 0.2 Āµm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Methods
Biotinylated Human SIRP alpha V2/CD172a Protein Tris-Bis PAGE
- Biotinylated Human SIRP alpha V2/CD172a on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Biotinylated Human SIRP alpha V2/CD172a Protein SEC-HPLC
- The purity of Biotinylated Human SIRP alpha V2/CD172a is greater than 95% as determined by SEC-HPLC.
Biotinylated Human SIRP alpha V2/CD172a Protein ELISA Data
- Immobilized Biotinylated Human SIRP alpha V2, His Tag at 0.5Ī¼g/ml (100Ī¼l/Well) on the plate. Dose response curve for Human CD47, hFc Tag with the EC50 of 0.11Ī¼ng/ml determined by ELISA.
