///Biotinylated HLA-A*02:01 LMLGEFLKL peptide complex

Biotinylated HLA-A*02:01 LMLGEFLKL peptide complex

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$210.00$850.00

SKU: KTW80VEE Tags: , ,

Description

Accession
A0A140T913
Source
DNA sequence encoding the Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M)and LMLGEFLKL peptide.This complex includes a C terminal C-His-Avi tag and was expressed in HEK cells.
Molecular weight
Recombinant HLA-A*02:01 LMLGEFLKL peptide complex is a glycosylated protein. The protein has a predicted MW of 50.5 kDa. Due to glycosylation, the protein migrates to 55-63 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant HLA-A*02:01 LMLGEFLKL peptide complex was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

Methods

Biotinylated Human Survivin (HLA-A*02:01) Protein SEC-HPLC

  • The purity of Biotinylated Human Survivin (HLA-A*02:01) Proteinwas greater than 95% as determined by SEC-HPLC.

Biotinylated Human Survivin (HLA-A*02:01) Protein Tris-Bis PAGE

  • Biotinylated Human Survivin (HLA-A*02:01) Protein PAGE under reduced condition. The purity is greater than 95%.
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