HLA-A*02:01 P53 R175H peptide complex Tetramer
Categories: HLA-A peptide complexesMHC peptide complexes$510.00 – $2,025.00
Description
Accession
P53
Source
DNA sequence encoding the Human (HLA-A*02:01) AA25-305 [accession# P04439] and the B2M protein AA2-119 [accession# P61769], fused to the mutant P53 R175H epitope HMTEVVRHC .This complex includes a C terminal polyHis AVI tag and was expressed in HEK cells, The teramer conformation was achieved through biotinylation of the monomer and then covalently binding the monomers via an avidin biotin reaction.
Molecular weight
Recombinant HLA-A/P53 R175H has a calculated mass of approximately 250kDa. Due to glycosylation migrates as an approximately 260 kDa protein under reducing conditions in SDS-PAGE.
Purity
purity was determined to be >95% by nonreducing PAGE and HPLC (see below methods)
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant HLA-A*02:01 P53 R175H was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Methods
HLA-A*02:01 P53 R175H peptide complex Trimer PAGE
- 1ug loaded Tris-Bis PAGE under reducing condition. The purity is greater than 95%.
HLA-A*02:01 P53 R175H complex Trimer HPLC
- The purity of P53 R175H (HLA-A*02:01)complex Trimer was greater than 95% as determined by SEC-HPLC.
HLA-A*02:01 P53 R175H complex Trimer peptide complex tetramer ELISA
- Dose response curve for Anti-HLA-A*02:01 P53 R175H Antibody
