Optimized DNA sequence encodingÂ Human Signal regulatory protein alpha - SIRP alpha Â (SHPS1)Â Â (GLU31 - ARG370) extracellular domain including a C-terminal His tag was expressed in HEK293 cells.
Recombinant Human SIRP alpha Â (SHPS1) is a protein consisting of 176 amino acid residue subunits,due to glycosylation migrates as an approximately as 35kDa band protein on reduced SDS-PAGE.
>95%, as determined by SDS-PAGE and HPLC
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/Âµg(1EU/Âµg).
Recombinant human SIRP alpha (SHPS1) is lyophilized Â from 0.2 Î¼m filtered PBS solution, Â pH7.2 , Â 5% Trehalose.
Â Â Â Â Â Â Â Â
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Recombinant Human SIRP alpha (SIRPA) can be stored in working aliquots at 2Â° - 8Â° C for one month, or at -20Â°C toÂ -70Â°CÂ for twelve months.
Avoid repeated freeze/thaw cycles.
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Signal regulatory protein alpha
Sirpa encodes an Ig
expressed on macrophages,
dendritic cells, and
neurons. SIRPα and its
ligand CD47 interact through
their respective Ig variable
region (IgV)–like domains .
Upon binding CD47, SIRPα
based inhibition motifs
mediate inhibitory signals
via recruitment of the src
homology-2 domain containing
phosphatases SHP-1 and SHP-2
leading to decreased
phagocytosis by macrophages,
inhibition of neutrophil
migration, and attenuated
production of the
inflammatory cytokine TNF
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natural killer cells in hiv controller patients express an activated effector phenotype and do not up-regulate NKp44 on il-2 stimulation
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kaposi's sarcoma-associated herpesvirus orf54/dutpase downregulates a ligand for the nk activating receptor NKp44 J. Virol.,
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proliferating cell nuclear antigen is a novel inhibitory ligand for the natural cytotoxicity receptor NKp44 J. Immunol.,
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gut inflammation and indoleamine deoxygenase inhibit il-17 production and promote cytotoxic potential in NKp44+ mucosal nk cells during siv infection
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