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Human SIRP alpha Recombinant

SHPS-1

accession:  P78324
   Size A     20ug   $ 160
   Size B      100ug  $480
   Size C      500ug  $ 1950
Domain  : 
Gene  :  SIRPA
Catalog no. :  RKP78324

 

Source:

Optimized DNA sequence encoding Human Signal regulatory protein alpha - SIRP alpha  (SHPS1)  (GLU31 - ARG370) extracellular domain including a C-terminal His tag was expressed in HEK293 cells.


 
Molecular weight:

Recombinant Human SIRP alpha  (SHPS1) is a protein consisting of 176 amino acid residue subunits,due to glycosylation migrates as an approximately as 35kDa band protein on reduced SDS-PAGE.


 
Purity:
>95%, as determined by SDS-PAGE and HPLC
 
 
 
Endotoxin:
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

 
Presentation:
Recombinant human SIRP alpha (SHPS1) is lyophilized  from 0.2 μm filtered PBS solution,  pH7.2 ,  5% Trehalose.

              
Reconstitution:
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

 
Storage:
Recombinant Human SIRP alpha (SIRPA) can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to -70°C for twelve months.

Avoid repeated freeze/thaw cycles.

 
Usage:
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

 

Signal regulatory protein alpha

Sirpa encodes an Ig superfamily receptor expressed on macrophages, dendritic cells, and neurons. SIRPα and its ubiquitously expressed ligand CD47 interact through their respective Ig variable region (IgV)–like domains . Upon binding CD47, SIRPα immunoreceptor tyrosine- based inhibition motifs mediate inhibitory signals via recruitment of the src homology-2 domain containing protein tyrosine phosphatases SHP-1 and SHP-2 leading to decreased phagocytosis by macrophages, inhibition of neutrophil migration, and attenuated production of the inflammatory cytokine TNF

Related Publications:
identification of a cellular ligand for the natural cytotoxicity receptor NKp44
Blood, Oct 2013; 122: 2935 - 2942.
natural killer cells in hiv controller patients express an activated effector phenotype and do not up-regulate NKp44 on il-2 stimulation
PNAS, Jul 2013; 110: 11970 - 11975.
kaposi's sarcoma-associated herpesvirus orf54/dutpase downregulates a ligand for the nk activating receptor NKp44
J. Virol., Aug 2012; 86: 8693 - 8704.
proliferating cell nuclear antigen is a novel inhibitory ligand for the natural cytotoxicity receptor NKp44
J. Immunol., Dec 2011; 187: 5693 - 5702.
gut inflammation and indoleamine deoxygenase inhibit il-17 production and promote cytotoxic potential in NKp44+ mucosal nk cells during siv infection
Blood, Sep 2011; 118: 3321 - 3330.




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